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. Author manuscript; available in PMC: 2005 Oct 5.
Published in final edited form as: Glycobiology. 2004 Dec 22;15(5):529–539. doi: 10.1093/glycob/cwi030

Figure 2. Effect of NEM on the activity of seHAS single Cys-to-Ser mutants.

Figure 2

Membranes containing wildtype seHAS (▴), or the C226S (•), C262S (▿), C281S (▪) or C367S (○) single Cys-mutants of seHAS were treated with 5 mM NEM at 4°C and the HAS activity of samples was determined at the indicated times as described in Methods. Panel B is a blowup of the early incubation times shown in Panel A. Results are the mean ± SEM for three separate experiments using different membrane preparations (n=3).