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. Author manuscript; available in PMC: 2005 Oct 5.
Published in final edited form as: Glycobiology. 2004 Dec 22;15(5):529–539. doi: 10.1093/glycob/cwi030

Figure 5. Reactivity of [14C]NEM with the Cys-to-Ala triple Cys-mutants of seHAS.

Figure 5

Equal amounts of E. coli membranes containing wildtype or a triple Cys-mutant of seHAS were treated with [14C]NEM and subjected to SDS-PAGE as described in Methods. The 367 lane, which showed no labeling with NEM, is identical to the result with the Cys-null seHAS mutant reported previously (Kumari et al., 2002).