Table 3.
Final concentration of elements in growth medium
| Element | Form added | Supplemented concentration | Final concentration |
| Calcium | CaCl2 | 1 mM | 1.2 mM |
| Cobalt | CoCl2 | 5 μM | 5.1 μM |
| Copper | CuCl2 | 100 μM | 110 μM |
| Iron | - | - | 19 μM |
| Magnesium | - | - | 490 μM |
| Manganese | MnCl2 | 10 μM | 11 μM |
| Nickel | NiCl2 | 125 μM | 160 μM |
| Potassium | - | - | 12 mM |
| Phosphorus | - | - | 5.7 mM |
| Selenium | Na2SeO3 | 75 μM | 75 μM |
| Sodium | - | - | 16 mM |
| Sulfur | - | - | 5.4 mM |
| Zinc | ZnCl2 | 100 μM | 160 μM |
Calcium, cobalt, copper, manganese, nickel, selenium and zinc were added to rich YPD medium (1% yeast extract, 2% peptone, 2% glucose) to facilitate their detection in cells by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). These supplemented levels did not exceed 10% of the minimal growth inhibitory concentration determined for this strain (data not shown). The final concentration of these elements in the growth medium measured by ICP-AES is also shown and represents the supplemented levels plus those present in the YPD medium alone. The same lots of all medium components (such as yeast extract, peptone) were used throughout this study to maintain consistent growth conditions. Boron was added as H2BO3 at 181 μM and molybdenum was added as NaMoO4 at 10 μM. Despite this supplementation, levels of these two minerals accumulated by cells remained below the level of detection. -, not supplemented.