Figure 2.

RNA Gel Blot with the Same Flower Bud RNAs Used to Hybridize to the Soybean Microarrays.

(A) RNA gel blot containing 10 micrograms of each RNA sample (cleaned by RNeasy Qiagen minicolumns) from flower buds of LN89-5322-2 (wp) and LN89-5320-6 (Wp) isolines. A 1.4-kb transcript is detected in the purple flower line (Wp) and is not visible in the pink flower (wp) isoline. The Gm-c1012-683 cDNA clone was used as probe.

(B) Ethidium bromide–stained gel prior to membrane transfer. The 25 S and 17 S rRNAs are shown to compare sample loading.