Figure 4.

RFLP Analysis of Soybean Lines Varying at the Wp Locus.

DNA gel blot of genomic DNA from five soybean lines digested with three different restriction enzymes, HindIII, BamHI, and EcoRI. The genotype and phenotype of each line (1 to 5) are described in Table 1. The type of Wp allele of each line is indicated at the top. The sizes of marker fragments are shown at the right in kilobases. The Gm-c1012-683 cDNA clone was used as probe. Arrows point to DNA polymorphisms at the Wp locus. A polymorphism detected in the RM lines (4 and 5; asterisk) is likely due to genetic diversity, since the RM lines are not isolines with lines 1 to 3 (Table 1). The bottom panel is a map of HindIII, BamHI, and EcoRI restriction sites for F3H1 and F3H2. The size of these genomic sequences is indicated in base pairs at the right. The sizes of HindIII and EcoRI fragments are given in kilobases.