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. 2005 Oct;17(10):2676–2692. doi: 10.1105/tpc.105.033415

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Copyright © 2005, American Society of Plant Biologists

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Figure 10. — Expression of Primary Auxin-Responsive Genes in IAA9-Inhibited Lines.

(A) RT-PCR analyses of the expression of auxin-responsive genes (IAA3, IAA6, IAA2, GH3, SAUR, and DR12) in the wild type and IAA9-inhibited (AS) line (AS213) upon auxin treatment (20 μM IAA for 3 h).

(B) Expression pattern of the auxin-inducible IAA3 promoter (Pro_IAA3) fused to the GUS reporter gene in wild-type seedlings. Inset contains enlarged picture showing GUS staining limited to small spots corresponding to the root tip and lateral root initiation sites (arrowheads).

(C) Expression pattern of Pro_IAA3:GUS in wild-type seedlings treated with exogenous auxin (20 μM IAA). Inset shows GUS staining throughout the root.

(D) Expression pattern of Pro_IAA3:GUS in AS-IAA9 background with no auxin treatment. Inset shows GUS staining present in all parts of the root system. Bars = 5 mm in (B) to (D).