Enhanced Auxin Sensitivity in IAA9-Inhibited Lines.
(A) Auxin dose–response assay of cotyledon explants. Root formation is induced by lower auxin (NAA) concentration in AS-IAA9 (AS) plants than in the wild type. Root regeneration is promoted at 0.1 μM NAA in the wild type and at 10 times lower concentration (0.01 μM) in AS-IAA9 lines.
(B) Auxin dose response in hypocotyl segments. Elongation is given as percentage increase in final length over the initial length after 23 h incubation in a solution containing the indicated NAA concentration. The results are representative of data obtained with three independent AS-IAA9 lines: AS213, AS111, and AS250. Error bars represent mean ± se, n ≥ 20.