Figure 4.

Spatial Expression Pattern of IAMT1.

The expression pattern of IAMT1 was determined using transgenic lines transformed with pIAMT1P-GUS, in which a GUS reporter gene was driven by the IAMT1 promoter.

(A) A 3-d-old seedling of the pIAMT1P-GUS reporter line.

(B) A 22-d-old seedling of the pIAMT1P-GUS reporter line.

(C) to (H) Rosette leaves of pIAMT1P-GUS reporter lines, from young to old. The expression of IAMT1 wanes from the midvein to the margin with the maturation of the leaf.

(I) Silique of the pIAMT1P-GUS reporter line.

(J) Close-up view of funiculus and seed from the pIAMT1P-GUS reporter line.

(K) Inflorescence and flower of the pIAMT1P-GUS reporter line.

(L) Analysis of IAMT1 expression level in leaf marginal and central sections by RT-PCR. RNA was extracted from the marginal or central section (dissected as shown at top) of leaves from 14-, 21-, and 28-d-old wild-type Arabidopsis. A DNA gel blot of RT-PCR products probed with cDNA of IAMT1 is shown. TUB2 was used as an internal control. The expression of IAMT1 is higher in the margin than in the central section and higher in young leaves than in old leaves.

Bars = 200 μm in (J) and 1 mm in all other panels.