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Expression Analysis of Leaf Curvature–Related Genes by RNA Gel Blot.
TCP3, TCP4, TCP10, TCP17, and TCP24 are homologous with CINCINNATA, which regulates leaf curvature in snapdragon (Nath et al., 2003). HASTY is another gene that results in the curly leaf phenotype when mutated (Telfer and Poethig, 1998). Except for TCP17, these genes were downregulated in iamt1-D. Ethidium bromide (EtBr) staining was used as the loading control.
