Table 2. Activity rhythms were altered in the Fmr1 KO mutants.
The locomotor activity rhythms of adult male WT and Fmr1 KO mice in the standard 12:12 hr LD cycles and constant darkness (DD) were monitored using wheel running activity (n = 6/group). Values are shown as the averages ± SEM. If the assumptions of normality and equal variance were met, a t-test was used to analyze the data; otherwise, the Mann–Whitney rank sum test was used. Asterisks indicate significant differences between genotypes. Alpha = 0.05. Degrees of freedom are reported between parentheses. Bold values indicate statistically significant differences.
| WT | Fmr1 KO | Statistical values | |
|---|---|---|---|
| LD | |||
| Rhythmic power (% variation) | 42.8 ± 1.7 | 30.4±3.8* | t(10)=3.309; p=0.008 |
| Cage activity (rev) | 19,875 ± 2197 | 24,302 ± 4294 | t(10)=−1.005; p=0.340 |
| Amplitude (rev) | 2553 ± 246 | 2771±406 | t(10)=−0.502; p=0.630 |
| Activity in the light phase (ZT 0–3, rev) | 418 ± 50.7 | 1099 ± 165* | U=0.000; p=0.002 |
| Onset variability (min) | 8.1 ± 2.0 | 24.5 ± 10.6 | t(10)=−2.060; p=0.066 |
| Fragmentation (bouts #) | 21.0 ± 0.6 | 24.2 ± 2.5 | U=12.000; p=0.390 |
| DD | |||
| Period (tau; hr) | 23.6 ± 0.1 | 23.4 ± 0.2 | t(10)=0.919; p=0.380 |
| Rhythmic power (% variation) | 47.4 ± 1.2 | 39.1 ± 2.0* | t(10)=5.319; p=0.003 |
| Cage activity (rev) | 27,225 ± 1495 | 27914 ± 3,218 | t(10)=−0.213; p=0.836 |
| Onset variability (min) | 16.3 ± 2.9 | 42.5 ± 7.9* | t(10)=−3.387; p=0.007 |
| Fragmentation (bouts #) | 21.3 ± 1.2 | 24.1±1.8 | t(10)=−1.416; p=0.187 |