Fig. 4.

Mutation (C→S) of the CaaX motif to prevent farnesylation of GFP-progerin and GFP-lamin A-UC reverses the nuclear morphology defects. (A) HeLa cells were transiently transfected with the indicated constructs and examined by fluorescence microscopy as in Fig. 2. Expected structures of the GFP-tagged proteins are shown. (B and C) Nuclei from HeLa cells transfected with the GFP-tagged constructs were scored for abnormal nuclear morphology (B) and nuclear envelope localization (C) of the GFP-tagged proteins as in Fig. 2. Graphs represent the average of two experiments with the range indicated by the bar.