Fig. 1.
Derlins interact with VIMP. (a) Interactions of US11WT, class I HC, Derlin-1, and VIMP. Immunoprecipitations from digitonin lysates of US11WT or US11Q192L cell lines pulse-labeled for 30 min and chased for the indicated times were performed with anti-Derlin-1 (lanes 1-4) or anti-VIMP (lanes 5-8) Abs. The positions of the relevant polypeptides are indicated. (b) VIMP associates with Derlin proteins. U373 cells were labeled to steady state, and immunoprecipitations from digitonin lysates were performed with control rabbit IgG (lane 1) or anti-VIMP Abs (lanes 2-5). The anti-VIMP immunoprecipitate (IP) was either analyzed directly (lane 2) or subjected to reimmunoprecipitation (re-IP) in sequential fashion by using the Abs indicated.