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. 2005 Aug 10;102(40):14142–14149. doi: 10.1073/pnas.0505018102

Fig. 3.

Fig. 3.

Regulation by RA of B cell distribution and cell division (CFSE dilution) elicited by BCR and CD38 ligation. Isolated B cells were labeled with CFSE and cultured for 5 days with and without stimuli (anti-μ or anti-CD38, each 1 μg/ml) and RA (10 nM). Cells were harvested on day 5 and analyzed for CFSE intensity by flow cytometry. (A) FALS versus side scatter plots were used to analyze the data. A representative panel of graphs of the distribution of B cells after treatment is shown. Three arbitrary gates (U, upper; M, middle; B, bottom) were made to divide the cell population according to the forward scatter. (B) Flow cytometric histograms of CFSE dilution in control and stimulated B cells with and without RA; the percentage of cells that had undergone dilution is indicated above the bars.