Fig. 7.
CD19 and sIgG1 expression during BCR and CD38 ligation-induced B cell activation. B cells were cultured for 5 days in the presence and absence of anti-μ plus anti-CD38 (1 μg/ml each) and RA (10 nM). At the time of harvesting each day, surface CD19 and sIgG1 were detected by anti-CD19-PE and anti-IgG1-Alexa 488 antibodies, respectively, and analyzed by flow cytometry. (A) The percentage of CD19+ cells during culture and after stimulation. Con, control; S±RA, stimuli in the presence and absence of RA (which did not differ). (B) The percentage of sIgG1+/CD19- cells. *, the difference between S and S+RA; **, the difference between S and Con. P < 0.05.