Table 2.
Wild-type blastocysts fail to implant in Fkbp52−/− recipients
| Genotype
|
No. of blastocysts transferred
|
No. of blastocysts recovered
|
|||||
|---|---|---|---|---|---|---|---|
| Blastocysts | Recipients | No. of recipients | No. of mice with IS (%) | No. of IS (%) | No. of IS | ||
| +/+ | +/+/+/− | 178 | 10 | 8 (80) | 74 (41) | 7.3 ± 4.0 | 2* |
| +/+ | −/− | 79 | 4 | 0 (0) | 0 | 0 | 14† |
Day 4 wild-type (+/+) blastocysts were transferred into uteri of +/+, +/−, or −/− recipients on day 4 of pseudopregnancy. The recipients were examined for implantation sites (IS) on day 5 by the blue dye method. Uteri without IS were flushed with saline to recover any unimplanted blastocysts.
Two mice without IS yielded one blastocyst each.
Fourteen blastocysts were recovered from three recipients. The results of +/+ and +/− mice were not significantly different and are presented as a pool (mean ± SD).