Skip to main content
. 2005 Sep 23;102(40):14314–14320. doi: 10.1073/pnas.0506475102

Fig. 3.

Fig. 3.

Activation of caspase-2, -3, and -7 in PIDD-expressing MEFs depends on RAIDD. Western blots were performed with the indicated antibodies on lysates prepared from E1A/Ras-transformed RAIDD+/+ and RAIDD-/- MEFs after infection with pMIG or pMIG-PIDD (A and B). The infection efficiency was determined by the proportion of cells that became GFP-positive 24 h after infection. The infection efficiency of RAIDD+/+ MEFs was 98% with pMIG and 91% with pMIG-PIDD; the infection efficiency of RAIDD-/- MEFs was 97% with pMIG and 92% with pMIG-PIDD. (C) Caspase activity assays were performed as described in Experimental Procedures. (Upper) The histograms show the fold increase in activity measured in RAIDD+/+ cells 48 h after infection with pMIG-PIDD compared with pMIG infection. (Lower) The change in activity at different time points after infection is shown.