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PI3P is required for mTOR signaling, and hVps34 associates with mTOR. (A) HeLa cells were transfected with 16 nM hVps15 siRNA and analyzed as in Fig. 4C.(B) HEK293 cells were transfected with myc-S6K1 and a GFP-FYVE expression vector and analyzed as in Fig. 4A.(C) Model depicting two signaling pathways converging on the mTOR/raptor/GβL signaling complex.
