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. 2025 Aug 4;17(9):2483–2524. doi: 10.1038/s44321-025-00281-9

Reagents and tools table

Reagent/resource Reference or source Identifier or catalogue number
Experimental models
Human left ventricular myocardium This study N/A
Antibodies
Alexa Flour 647-conjugated wheat germ agglutinin Molecular Probes W32466
Alexa Fluor 488 donkey anti-rabbit IgG Thermo Fisher Scientific ab15065
Anti-ACTN2 mouse IgG Sigma-Aldrich A7732
Anti-ALDOA rabbit IgG Sigma-Aldrich HPA004177
Anti-ATP2A2/SERCA2 mouse IgG Thermo Fisher Scientific MA3919
Anti-GFM1 rabbit IgG Sigma-Aldrich HPA061405
Anti-NDUFS3 rabbit IgG Abcam ab177471
Anti-TNNI3 (cardiac troponin I) rabbit IgG Abcam ab47003
DyLight 550 IgG donkey anti-mouse IgG Abcam ab9879
Chemicals, enzymes, and other reagents
Acetonitrile (HPLC grade) Thermo Fisher Scientific A955-4
Ammonium formate Sigma-Aldrich 70221
Chloroacetamide Sigma-Aldrich C0267
Chloroform (HPLC grade) Thermo Fisher Scientific C606SK-1
DAPI Thermo Fisher Scientific 62248
DPX Sigma-Aldrich 06522
Formic acid (LC-MS grade) Thermo Fisher Scientific A117-50
Methanol (HPLC grade) Thermo Fisher Scientific A456-4
Mito-ID Enzo Life Sciences ENZ-51022-0100
Pierce™ BCA Protein Assay Thermo Fisher Scientific 23225
ProLong Diamond antifade mountant Thermo Fisher Scientific P36970
Qubit™ dsDNA High Sensitivity Quantification Assay Kit Thermo Fisher Scientific Q32851
Sodium deoxycholate Sigma-Aldrich 30970
Superfrost Plus slides Menzel, Thermo Fisher Scientific J1810AMNZ
TCEP Thermo Fisher Scientific 20490
Tris-HCl pH 7.5 Sigma-Aldrich T5941
Triton X-100 Sigma-Aldrich T8787
TRIzol Invitrogen 15596026
Trypsin (sequencing grade) Promega V5111
Van Gieson’s stain This study N/A
Verhoeff’s Iron Haematoxylin solution This study N/A
Software
Adobe Illustrator Adobe Version 27.8
BioRender https://biorender.com N/A
clusterProfiler https://bioconductor.org/packages/clusterProfiler Version 4.8.1
DeepVenn https://www.deepvenn.com/ N/A
DIA-NN https://github.com/vdemichev/DiaNN Version 1.7
Gene Ontology (GO) https://geneontology.org/ Version 3.17.0
ggplot2 https://github.com/tidyverse/ggplot2 Version 3.4.2
GraphPad Prism GraphPad Version 9.5.1
Huygens Professional Scientific Volume Imaging Version 22.10.0p1
ImageJ ImageJ Version 1.54f
KEGG https://www.genome.jp/kegg/ Version 102.0
KEGGREST https://github.com/Bioconductor/KEGGREST Version 1.40.0
limma https://github.com/gangwug/limma Version 3.56.2
LipidSearch Thermo Fisher Scientific Version 4.2
Microsoft Excel Microsoft Version 2309
MitoCarta3.0 Rath et al, (2021) N/A
NIS-elements Nikon Version 4.0
PANTHER http://www.pantherdb.org Version 17.0
R R Version 4.3.1
Rsubread https://bioconductor.org/packages/release/bioc/html/Rsubread.html Version 2.2.1
SCIEX OS AB Sciex Version 1.7.0.36606
scMerge https://github.com/SydneyBioX/scMerge Version 1.16.0
SRplot https://www.bioinformatics.com.cn/en N/A
STRING https://string-db.org Version 12.0
ZEN Blue Zen Version 3.3
Other
Agilent 1260 Infinity LC system Agilent Technologies N/A
Agilent High Sensitivity D1000 Tapestation system Agilent N/A
Atlantis® HILIC column Waters N/A
Axio Scan.Z1 Zeiss N/A
Bioanalyzer Agilent N/A
C18 analytical column (1.9 μm) Dr. Maisch GmbH N/A
C18 HPLC column Thermo Fisher Scientific N/A
Cryotome FSE Thermo Fisher Scientific N/A
LabChip GX Touch Revvity N/A
NanoDrop Thermo Fisher Scientific N/A
Nikon C2plus confocal microscope Nikon N/A
NovaSeq 6000 Illumina N/A
Q-Exactive Fusion Lumos MS Thermo Fisher Scientific N/A
Q-Exactive HF-X MS Thermo Fisher Scientific N/A
QTRAP5500 Mass Spectrometer AB Sciex N/A
Savant SC210 SpeedVac Thermo Fisher Scientific N/A
Shiny App (Human-Heart-ALL) The University of Sydney http://shiny.maths.usyd.edu.au/Human-Heart-ALL/
SpeedVac SPD120 Thermo Fisher Scientific N/A
ThermoMixerC Eppendorf N/A
TissueLyser LT Qiagen N/A
XBridge™ Amide column Waters N/A