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. 2005 Aug 30;2:11. doi: 10.1186/1743-1050-2-11

Figure 1.

Figure 1

Detection of germ line variants in NRIP1 in patients with severe endometriosis. A) Spectrofluorimetric analysis of NRIP1 gene using real-time PCR. Analysis of the fluorescence measured during melting curve determination in the LightCycler (Roche Applied Science). Each allele has a specific melting point and all alleles are represented by its specific nucleotide change with the exception of Ile441Val and Arg448Gly polymorphisms. Nt c.512 G->A (Gly75Gly, melting points, Allele G: 62°C; Allele A: 57°C). Nt c.949 A->G (His221Arg, melting points, Allele A 61°C; Allele G: 56°C). Nt c.1608 A->G (Ile441Val) and Nt c.1629 C->G (Arg448Gly) (melting points, Allele Val441: 59°C; Allele Gly448: 56°C; wild type: 63°C). Nt c.2695 C->T (Ser803Leu, melting points, Allele C: 61°C; Allele T: 55°C). Nt c.3522 G->T (Val1079Phe, melting points, Allele G: 62°C; Allele T: 54°C). B) Sequence conservation and location of mutations in the RIP-140 protein. Black shading indicates the position of mutations. LXXLL motifs responsible for ligand independent interaction with Retinoid Acid Receptor (RAR) and Retinoid X Receptor (RXR) are in bold and underlyned. Signal peptide is depicted in blue, Carboxyl terminal binding protein (CTBP) and RAR interacting motifs are in red and green respectively. Low complexity regions are shown in grey.