Table 2.
Anchor and Sensor probes sequences employed for coding Single Nucleotide Polymorphism (cSNP) analysis using Fluorescence Resonance Energy Transfer (FRET) technology.
| Mutation | PROBE | SEQUENCE 5' → 3 |
| Gly75Gly | 75-ANCHOR | AGTAATGGTCCAGTTCTCAATACAC – F |
| 75-SENSOR | Cy5 – TACATATCAGGGGTCTGGC – Ph | |
| His221Arg | 221-ANCHOR | Cy5 – AGTGGAACAAAGGTCATGAGTGAAC – Ph |
| 221-SENSOR | TCTCCTCATCATGTTGGACA – F | |
| Ile441Val | 448-ANCHOR | TATTCCAACTGTGTTCCCATAGACT – F |
| Arg448Gly | 448-SENSOR | Cy5 – GTCTTGCAAACACCGAACTG – Ph |
| Ser803Leu | 803-ANCHOR | GCGCACCTGCCTTACCAGTGTCCCGA – F |
| 803-SENSOR | Cy5-GACTTTAAATCGGAGCCTGTT – Ph | |
| Val1079Phe | 1079-ANCHOR | Cy5 – CGAGAAACACAAGACAAGGACATTT – Ph |
| 1079-SENSOR | GGAGGCAATTCTGTTACCAG – F |
Nomenclature: F: Fluoresceine, Ph: Phosphate.