Skip to main content
. 2002 May 21;99(11):7390–7395. doi: 10.1073/pnas.112085599

Figure 3.

Figure 3

ATPase activity of LolCDE reconstituted into proteoliposomes. (A) Proteoliposomes were reconstituted with LolCDE alone (open circles), Pal (9. 2 nmol) and LolCDE (filled circles), or Pal(D) (9.2 nmol) and LolCDE (filled triangles). ATP hydrolysis by these proteoliposomes was determined as described under Materials and Methods. Where specified, LolCD(S147N)E was reconstituted in place of LolCDE (filled squares), or the reaction mixture contained 10 mM ortho-vanadate (open triangles). (B) The ATPase activity of proteoliposomes reconstituted with LolCDE, and various amounts of Pal or Pal(D) was determined. Note that a lower part of the axis is deleted. (C) Proteoliposomes reconstituted with LolCDE and Pal (3.4 nmol) were subjected to the release assay in the presence of LolA.