Figure 4.

Extracts from a [Het-s] strain containing HET-s aggregates convert soluble recombinant HET-s into aggregates. Recombinant soluble HET-s protein (1 mg⋅ml⁻¹) was inoculated with an equal amount (wt/wt) of a sucrose pellet fraction from a [Het-s] strain containing HET-s aggregates ([Het-s] lane), or from the het-s-knockout strain (Δhet-s lane), or with buffer A alone (“no extract” lane) and kept at 4°C. The reaction mixture was centrifuged at 10,000 × g for 15 min after 30 min, 12 h, and 26 h, and supernatant and pellet fractions were analyzed by SDS/PAGE and Coomassie blue staining. The position of recombinant HET-s protein, which migrates at 32 kDa, is marked.