Table 2.
Centrifugation speed (× g) | 100,000 | 10,000 | 2,000 | |||
---|---|---|---|---|---|---|
Fractions | S | P | S | P | S | P |
Amount of HET-s protein | 2.7 | 3 | 1.6 | 2.3 | 1 | 2.6 |
No. of [Het-s] strains | 7/132 | 54/60 | 38/224 | 105/144 | 21/32 | 59/69 |
% of [Het-s] induction | 5 | 90 | 17 | 73 | 67 | 86 |
The first line gives the centrifugation speed (in g). The second line gives the amount of the protein used in micrograms per mycelium for the supernatant (S) and the pellet (P) fractions. The third line gives the number of strains that acquired the [Het-s] prion over the total number of strains and the corresponding percentage of [Het-s] induction.