Figure 4.

Stereo ribbon diagram of the hypothetical model of complex formed between proMMP-2, TIMP-2, and the catalytic domain of MT1-MMP. In the model shown, TIMP-2 is a hybrid with its C-terminal domain taken from the presented proMMP-2/TIMP-2 complex structure (magenta) and the N-terminal TIMP-2 half (red) is obtained from the model of the MT1-MMP/TIMP-2 inhibitory complex (Protein Data Bank code 1BUV) (26). Such combining was done because of the structural differences between complexed and uncomplexed forms of TIMP-2 molecules. Coloring for proMMP-2: the propeptide is pink, catalytic domain is blue, three fibronectin-like domains are green, and the hemopexin domain is yellow; for MT1-MMP: the catalytic domain is light blue.