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. 2002 May 28;99(11):7420–7425. doi: 10.1073/pnas.112008599

Figure 3.

Figure 3

Affinity purification of human BHC from Flag-BRAF35 cells. (a) Purification scheme. Nuclear extract (NE) from FBRAF35-4 cells was fractionated by using an anti-Flag M2 affinity column. Bound proteins were analyzed further by chromatography on a heparin-5PW column. (b) Silver-stain analysis of the anti-Flag affinity eluates (15 μl) corresponding to early (lane 1) and late (lane 2) eluting fractions. (c) Western blot, HDAC activity, and silver-stain analysis of the heparin-5PW column fractions (15 μl). Western blotting was performed by using antibodies shown to the right of the figure.