Co-trafficking of TRPC1 and TRPC6Δ131 by members of the TRPC family. C-terminally YFP-tagged hTRPC1A was expressed in HEK293 cells alone (a) or together with untagged hTRPC3 (b) or mTRPC4β (c), and the cellular localization was assessed by confocal laser-scanning microscopy. d shows a cell expressing TRPC6-GFP. An N-terminally truncated TRPC6 mutant C-terminally tagged with GFP (TRPC6Δ131-GFP) was expressed alone (e) or together with untagged hTRPC3 (f), hTRPC6 (g), mTRPC4β (h), or mTRPC5 (i). Typical examples from three independent transfections are shown. ne, nuclear envelope; pm, plasma membrane.