Figure 6.

Expression of signaling pathway proteins in TNBC cells following nintedanib treatment. (A) Western blot analysis of key signaling pathway proteins after nintedanib treatment. (B) Semi-quantification of relative protein expression levels. (C) Western blot analysis of EMT regulatory protein expression in TNBC cells after nintedanib treatment. (D) Statistical analysis of relative EMT protein expression levels. All data are presented as the mean ± SD from three independent experiments. ^*P<0.05, ^**P<0.01, ^***P<0.001 and ^****P<0.0001 vs. control. EMT, epithelial-mesenchymal transition; mTOR, mammalian target of rapamycin; p-, phosphorylated; PPARα, peroxisome proliferator-activated receptor α; TNBC, triple-negative breast cancer; TP73, tumor protein p73; ZEB, zinc finger E-box-binding homeobox protein.