Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J | The Jackson Laboratory | Jax Strain #000664 RRID:IMSR_JAX:000664 |
|
| Strain, strain background (Mus musculus) | Rosa26mTmG | The Jackson Laboratory | Jax Strain #007676 B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP) Luo/J RRID:IMSR_JAX:007676 |
|
| Strain, strain background (Mus musculus) | PdgfrαCre | The Jackson Laboratory | Jax Strain #013148 C57BL/6-Tg(Pdgfra-cre)1Clc/J RRID:IMSR_JAX: 013148 |
|
| Strain, strain background (Mus musculus) | Csfr1-EGFP | The Jackson Laboratory | Jax Strain #018549 B6.Cg-Tg(Csf1r-EGFP)1Hume/J RRID:IMSR_JAX:018549 |
|
| Strain, strain background (Mus musculus) | PdgfrαCreERTM | The Jackson Laboratory | Jax Strain #018280 B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J RRID:IMSR_JAX:018280 |
|
| Strain, strain background (Mus musculus) | Cspg4-Cre | The Jackson Laboratory | Jax Strain #:008533 B6;FVB-Ifi208Tg(Cspg4-cre)1Akik/J RRID:IMSR_JAX:008533 |
|
| Strain, strain background (Mus musculus) | ChR2/tdTomato | The Jackson Laboratory | Jax Strain #012567 B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J RRID:IMSR_JAX:012567 |
|
| Strain, strain background (Mus musculus) | PdgfrβCreERT2 | The Jackson Laboratory | Jax Strain #029684 B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J RRID:IMSR_JAX:029684 |
|
| Strain, strain background (Mus musculus) | Myh11CreERT2 | The Jackson Laboratory | Jax Strain #019079 B6.FVB-Tg(Myh11-icre/ERT2)1Soff/J RRID:IMSR_JAX:019079 |
|
| Strain, strain background (Mus musculus) | CkitCreERT2 | Dieter Saur (Technical University of Munich) | Kittm1(cre/ERT2)Dsa | |
| Strain, strain background (Mus musculus) | Prox1-eGFP | Young-Kwon Hong (University of Southern California) | MMRRC ID #31006 Tg(Prox1-EGFP)KY221Gsat/Mmucd RRID:MMRRC_031006-UCD |
|
| Strain, strain background (Mus musculus) | GCaMP6f | Jax | Jax Strain #028865 Ai95(RCL-GCaMP6f)-D (C57BL/6J) or Ai95D (C57BL/6J) RRID:IMSR_JAX:028865 |
|
| Antibody | anti-ACTA2 | Sigma-Aldrich | Cat# A2547, RRID:AB_476701 | (IF) 1:500 |
| Antibody | anti-GFP | Thermo Fisher Scientific | Cat# A-11122, RRID:AB_221569 | (IF) 1:200 |
| Antibody | anti-CKIT | Cell Signaling | Cell Signaling Technology Cat# 3074, RRID:AB_1147633 |
(IF) 1:100 |
| Antibody | anti-VIMENTIN | Thermo Fisher Scientific | Cat# OMA1-06001, RRID:AB_325529 | (IF) 1:100 |
| Antibody | anti-DESMIN | Invitrogen | Cat# PA5-16705, RRID:AB_10977258 | (IF) 1:200 |
| Antibody | anti-GFP | Abcam | Cat# ab13970, RRID:AB_300798 | (IF) 1:200 |
| Antibody | anti-CD34 (RAM34) | Thermo Fisher Scientific | Cat# 14-0341-82, RRID:AB_467210 | (IF) 1:200 |
| Antibody | anti-PDGFRα | R&D Systems | Cat# AF1062, RRID:AB_2236897 | (IF) 1:200 |
| Antibody | anti-PDGFRβ | eBiosciences | Cat# 14-1402-82, RRID:AB_467493 | (IF) 1:200 |
| Antibody | anti-CALPONIN | Abcam | Cat# ab46794, RRID:AB_2291941 | (IF) 1:500 |
| Antibody | anti-LY6A | Biolegend | Cat# 108101, RRID:AB_313338 | (IF) 1:200 |
| Sequence-based reagent |
Prox1- Forward NM_008937 |
NIH Primer Tool, this paper |
PCR primers | GTA AGA CAT CAC CGC GTG C |
| Sequence-based reagent |
Prox1- Reverse NM_008937 |
NIH Primer Tool, this paper | PCR primers | TCA TGG TCA GGC ATC ACT GG |
| Sequence-based reagent |
Itgam Reverse NM_008401 |
MGH Primer Bank ID 668048a1 |
PCR primers | ATG GAC GCT GAT GGC AAT ACC |
| Sequence-based reagent |
Itgam Forward NM_008401 |
MGH Primer Bank ID 668048a1 |
PCR primers | TCC CCA TTC ACG TCT CCC A |
| Sequence-based reagent |
Pdgfra Forward NM_011058 |
MGH Primer Bank ID 26349287a1 |
PCR primers | AGA GTT ACA CGT TTG AGC TGT C |
| Sequence-based reagent |
Pdgfra Reverse NM_011058 |
MGH Primer Bank ID 26349287a1 |
PCR primers | GTC CCT CCA CGG TAC TCC T |
| Sequence-based reagent |
Myh11 Forward NM_013607 |
MGH Primer Bank ID 7305295a1 |
PCR primers | AAG CTG CGG CTA GAG GTC A |
| Sequence-based reagent |
Myh11 Reverse NM_013607 |
MGH Primer Bank ID 7305295a1 |
PCR primers | CCC TCC CTT TGA TGG CTG AG |
| Sequence-based reagent |
Ckit Forward NM_021099 |
Drumm et al., 2018 | PCR primers | CGC CTG CCG AAA TGT ATG ACG |
| Sequence-based reagent |
Ckit Reverse NM_021099 |
Drumm et al., 2018 | PCR primers | GGT TCT CTG GGT TGG GGT TGC |
| Sequence-based reagent |
Pdgfrb Forward NM_008809 |
Basciani et al., 2004 | PCR primers | AGC TAC ATG GCC CCT TAT GA |
| Sequence-based reagent |
Pdgfrb Reverse NM_008809 |
Basciani et al., 2004 | PCR primers | GGA TCC CAA AAG ACC AGA CA |
| Sequence-based reagent |
Cdh5 Forward NM_009868 |
IDT Primer Quest Tool, this paper | PCR primers | CTT CCT TAC TGC CCT CAT TGT |
| Sequence-based reagent |
Cdh5 Reverse NM_009868 |
IDT Real time primer tool, this paper | PCR primers | CTG TTT CTC TCG GTC CAA GTT |
| Sequence-based reagent |
Nos3 Forward NM_008713 |
IDT Real time primer tool, this paper | PCR primers | CTG CCA CCT GAT CCT AAC TTG |
| Sequence-based reagent |
Nos3 Reverse NM_008713 |
IDT Real time primer tool, this paper | PCR primers | CAG CCA AAC ACC AAA GTC ATG |
| Sequence-based reagent |
Acta2 (Smooth Muscle Actin) Forward NM_007392 |
IDT TaqMan Mm.PT.58.16320644 | PCR primers | GAG CTA CGA ACT GCC TGA C |
| Sequence-based reagent |
Acta2 (Smooth Muscle Actin) Reverse NM_007392 |
IDT TaqMan Mm.PT.58.16320644 | PCR primers | CTG TTA TAG GTG GTT TCG TGG A |
| Sequence-based reagent |
Cacna1c (CaV 1.2) Forward NM_001159533 |
Cheng et al., 2007 | PCR primers | ATG GTC AAT GAA AAC ACG AGG ATG |
| Sequence-based reagent |
Cacna1c (CaV 1.2) Reverse NM_001159533 |
Cheng et al., 2007 | PCR primers | GGA ACT GAC GGT AGA GAT GGT TGC |
| Sequence-based reagent |
Cd34 Forward NM_133654 |
IDT Mm.PT.58.8626728 | PCR primers | GGT ACA GGA GAA TGC AGG TC |
| Sequence-based reagent |
Cd34 Reverse NM_133654 |
IDT Mm.PT.58.8626728 | PCR primers | CGT GGT AGC AGA AGT CAA GT |
| Sequence-based reagent |
Cspg4 (Ng2) Forward NM_139001 |
IDT Mm.PT.58.29461721 | PCR primers | CTT CAC GAT CAC CAT CCT TCC |
| Sequence-based reagent |
Cspg4 (Ng2) Reverse NM_139001 |
IDT Mm.PT.58.29461721 | PCR primers | CCC GAA TCA TTG TCT GTT CCC |
| Sequence-based reagent |
Vimentin Forward NM_011701 |
Li et al., 2016 | PCR primers | CTG TAC GAG GAG GAG ATG CG |
| sequence-based reagent |
Vimentin Reverse NM_011701 |
Li et al., 2016 | PCR primers | AAT TTC TTC CTG CAA GGA TT |
| Sequence-based reagent |
Desmin Forward NM_010043 |
MGH Primer Bank ID 33563250a1 |
PCR primers | GTG GAT GCA GCC ACT CTA GC |
| Sequence-based reagent |
Desmin Reverse NM_010043 |
MGH Primer Bank ID 33563250a1 |
PCR primers | TTA GCC GCG ATG GTC TCA TAC |
| Sequence-based reagent |
Mcam Forward NM_023061 |
MGH Primer Bank ID 10566955a1 |
PCR primers | CCC AAA CTG GTG TGC GTC TT |
| Sequence-based reagent |
Mcam Reverse NM_023061 |
MGH Primer Bank ID 10566955a1 |
PCR primers | GGA AAA TCA GTA TCT GCC TCT CC |
| Sequence-based reagent |
Klf4 Forward NM_010637 |
Majesky et al., 2017 | PCR primers | ATT AAT GAG GCA GCC ACC TG |
| Sequence-based reagent |
Klf4 Reverse NM_010637 |
Majesky et al., 2017 | PCR primers | GGA AGA CGA GGA TGA AGC TG |
| Sequence-based reagent |
Ly6a Forward NM_001271416 |
Majesky et al., 2017 | PCR primers | CTC TGA GGA TGG ACA CTT CT |
| Sequence-based reagent |
Ly6a Reverse NM_001271416 |
Majesky et al., 2017 | PCR primers | GGT CTG CAG GAG GAC TGA GC |
| Sequence-based reagent |
Gli1 Forward NM_01029 |
Kramann et al., 2015 | PCR primers | ATC ACC TGT TGG GGA TGC TGG AT |
| Sequence-based reagent |
Gli1 Reverse NM_01029 |
Kramann et al., 2015 | PCR primers | CGT GAA TAG GAC TTC CGA CAG |
| Sequence-based reagent |
Itgb1 Forward NM_010578 |
NIH Primer Tool, this paper | PCR primers | TCG ATC CTG TGA CCC ATT GC |
| Sequence-based reagent |
Itgb1 Reverse NM_010578 |
NIH Primer Tool, this paper | PCR primers | AAC AAT TCC AGC AAC CAC GC |
| Sequence-based reagent |
Eng Forward NM_007932 |
NIH Primer Tool, this paper | PCR primers | TGA GCG TGT CTC CAT TGA CC |
| Sequence-based reagent |
Eng Reverse NM_007932 |
NIH Primer Tool, this paper | PCR primers | GGG GCC ACG TGT GTG AGA A |
| Sequence-based reagent |
Cd44 Forward NM_009851 |
IDT Mm.PT.58.12084136 | PCR primers | CAC CAT TTC CTG AGA CTT GCT |
| Sequence-based reagent |
Cd44 Reverse NM_009851 |
IDT Mm.PT.58.12084136 | PCR primers | TCT GAT TCT TGC CGT CTG C |
| Sequence-based reagent |
Pecam1 Forward NM_008816 |
MGH Primer Bank ID 6679273a1 |
PCR primers | CTG CCA GTC CGA AAA TGG AAC |
| Sequence-based reagent |
Pecam1 Reverse NM_008816 |
MGH Primer Bank ID 6679273a1 |
PCR primers | CTT CAT CCA CTG GGG CTA TC |
| Sequence-based reagent |
Gjc1 Forward NM_008122 |
IDT Mm.PT.58.8383900 | PCR primers | GGT AAC AGG AGT TCT GGT GAA |
| Sequence-based reagent |
Gjc1 Reverse NM_008122 |
IDT Mm.PT.58.8383900 | PCR primers | TCG AAA GAC AAT CAG CAC AGT |
| Sequence-based reagent |
Anoctamin 1 Forward NM_178642 |
IDT Real time primer tool, this paper | PCR primers | GGC ATT TGT CAT TGT CTT CCA G |
| Sequence-based reagent |
Anoctamin 1 Reverse NM_178642 |
IDT Real time primer tool, this paper | PCR primers | TCC TCA CGC ATA AAC AGC TC |
| Sequence-based reagent |
Ptprc Forward NM_001111316 |
NIH Primer Tool, this paper | PCR primers | ATG CAT CCA TCC TCG TCC AC |
| Sequence-based reagent |
Ptprc Reverse NM_001111316 |
NIH Primer Tool, this paper | PCR primers | TGA CTT GTC CAT TCT GGG CG |
| Chemical compound, drug | Methylene Blue | Sigma | M9140 | |
| Chemical compound, drug | Blockaid | Thermo Fisher | A11122 | |
| Peptide, recombinant protein | Collagenase H | Sigma | C8051 | |
| Peptide, recombinant protein | Collagenase F | Sigma | C7926 | |
| Chemical compound, drug | Dithioerythritol (DTT) | Sigma | D8161 | |
| Peptide, recombinant protein | Elastase | Worthington | LS00635 | |
| Peptide, recombinant protein | Papain | Sigma | P4762 | |
| Antibody | Donkey anti-mouse AF647 | Thermo Fisher | A32787 | (IF) 1:200 |
| Antibody | Donkey anti-Rat AF555 | Thermo Fisher | A48270 | (IF) 1:200 |
| Antibody | Donkey anti-Rabbit AF488 | Thermo Fisher | A21206 | (IF) 1:200 |
| Antibody | Donkey anti-Goat AF647 | Thermo Fisher | A21447 | (IF) 1:200 |
| Antibody | Donkey anti-Goat AF555 | Thermo Fisher | A21432 | (IF) 1:200 |
| Commercial assay or kit | Arcturus PicoPure RNA isolation kit | Thermo Fisher | KIT0204 | |
| Software, algorithm | Prism 10 | GraphPad | ||
| Software, algorithm | Volumetry software (version G8d) | Grant Hennig | Drumm et al., 2017; Drumm et al., 2019a | |
| Software, algorithm | Seurat v4,v5 | RRID:SCR_016341 | Hao et al., 2021; Hao et al., 2024 | |
| Software, algorithm | FIJI BIOP-JACoP | Bolte and Cordelières, 2006 |