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Effect of NPC binding proteins on import of GFP-ERK2. Import assay (A) and quantitation (B) using GFP-ERK2 (0.8 μM, 50 μg/ml) was performed in REF52 cells in the presence of energy mix and the indicated amounts of karyopherin-β1 or NTF2/p10. (Magnification: A, ×63.)
