Table 2.
Amplification of cDNAs by the real-time reverse transcription-polymerase chain reaction.
| Step | Time | Temperature |
|---|---|---|
| Initial Denaturation | 2 minutes | 95ºС |
| Denaturation | 10 seconds | 94ºС |
| Annealing | 15 seconds | 60ºС |
| Extension | 30 seconds | 72ºС |
| 40 cycles | ||
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