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. 2002 May 14;99(11):7530–7535. doi: 10.1073/pnas.112209199

Figure 2.

Figure 2

Delimitation of candidate genomic region of Spl7. (A) Genetic linkage map of 298 F2 plants showing the relative position of Spl7 with the RFLP markers on chromosome 5. The numbers under the linkage map indicate the number of recombinants in the adjacent marker intervals. (B) YAC (Y) and PAC (P) clone contigs spanning the Spl7 region. Squares indicate YAC/PAC-end clones. Circles indicate STSs generated from the YAC/PAC-end sequences. (C) Fine-scale, high-resolution genetic linkage map of the Spl7 region developed from the analysis of 2,944 F2 plants. The numbers under the linkage map indicate the number of recombinants in the adjacent marker intervals. (D) Fine-scale genetic and physical map of the 16-kb Spl7 region. a to j indicate the CAPS markers. The estimated positions of recombination are shown by arrows. (E) The ORFs in the Spl7 candidate region predicted by GENSCAN. A 5.6-kb NspV-BglII wild-type genomic fragment containing the entire Spl7 candidate ORF was used in the complementation analysis.