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Expression analysis of Spl7 by RT-PCR assay. (A) Change in expression with growth stage. Plants were grown in the field, and leaves were collected every 14 days after sowing (DAS) for RNA extraction. Actin primers were used in the control amplification. (B) Change in expression with heat stress. Plants were grown in a growth chamber for 6 weeks (26°C, control) and then were incubated at 35°C for 1 day and at 42°C for 1 day more.
