Skip to main content
NCBI home page
Annals of Surgery logoLink to Annals of Surgery
. 1993 Apr;217(4):397–403. doi: 10.1097/00000658-199304000-00012

Collagen synthesis in intact skin is suppressed during wound healing.

L Ihlberg 1, K Haukipuro 1, L Risteli 1, A Oikarinen 1, M I Kairaluoma 1, J Risteli 1
PMCID: PMC1242807  PMID: 8466311

Abstract

OBJECTIVE: Simultaneous monitoring of total collagen synthesis as well as synthesis in intact skin and in the wound to verify the higher priority of wound healing after surgery. SUMMARY BACKGROUND DATA: Synthesis of acute phase proteins is stimulated by surgical trauma. At the same time, production of albumin is inhibited and there is a net catabolism of skeletal muscle proteins. Similarly, the authors have found a transient inhibition of total collagen synthesis after surgery. The authors hypothesized a lower priority in synthesis of structural and peripheral collagen for the benefit of wound healing. METHODS: The concentrations of the carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type III procollagen (PIIINP) were measured in suction blister fluid of intact skin and in wound fluid in ten surgical patients. PICP and PIIINP concentrations in serum were also measured. Specific radioimmunoassays were used. RESULTS: In peripheral skin, the median preoperative concentrations of PICP and PIIINP were 228 and 140 micrograms/L, respectively. On postoperative days 1, 2, 4 and 7, the median concentration of PICP was 145 (p = 0.01, Wilcoxon signed rank sum test), 102 (0.02), 159 (0.03), and 152 (0.06) micrograms/L, respectively. The postoperative medians of PIIINP were 68 (p = 0.17), 76 (0.04), 66 (0.06), and 56 (0.03) micrograms/L, respectively. At the same time, collagen synthesis in the wound increased dramatically from the second day on. After an initial decrease, propeptide concentrations in serum gradually increased from the fourth day on. CONCLUSIONS: Collagen synthesis is regulated for the benefit of the wound during the acute phase response.

Full text

PDF
397

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark R. A. Wound repair. Curr Opin Cell Biol. 1989 Oct;1(5):1000–1008. doi: 10.1016/0955-0674(89)90072-0. [DOI] [PubMed] [Google Scholar]
  2. Di Padova F., Pozzi C., Tondre M. J., Tritapepe R. Selective and early increase of IL-1 inhibitors, IL-6 and cortisol after elective surgery. Clin Exp Immunol. 1991 Jul;85(1):137–142. doi: 10.1111/j.1365-2249.1991.tb05694.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Diegelmann R. F., Lindblad W. J., Cohen I. K. A subcutaneous implant for wound healing studies in humans. J Surg Res. 1986 Mar;40(3):229–237. doi: 10.1016/0022-4804(86)90156-3. [DOI] [PubMed] [Google Scholar]
  4. Ford H. R., Hoffman R. A., Wing E. J., Magee D. M., McIntyre L., Simmons R. L. Characterization of wound cytokines in the sponge matrix model. Arch Surg. 1989 Dec;124(12):1422–1428. doi: 10.1001/archsurg.1989.01410120068014. [DOI] [PubMed] [Google Scholar]
  5. Goodson W. H., 3rd, Hunt T. K. Development of a new miniature method for the study of wound healing in human subjects. J Surg Res. 1982 Nov;33(5):394–401. doi: 10.1016/0022-4804(82)90054-3. [DOI] [PubMed] [Google Scholar]
  6. Granstein R. D., Deak M. R., Jacques S. L., Margolis R. J., Flotte T. J., Whitaker D., Long F. H., Amento E. P. The systemic administration of gamma interferon inhibits collagen synthesis and acute inflammation in a murine skin wounding model. J Invest Dermatol. 1989 Jul;93(1):18–27. doi: 10.1111/1523-1747.ep12277336. [DOI] [PubMed] [Google Scholar]
  7. Haukipuro K., Melkko J., Risteli L., Kairaluoma M. I., Risteli J. Connective tissue response to major surgery and postoperative infection. Eur J Clin Invest. 1992 May;22(5):333–340. doi: 10.1111/j.1365-2362.1992.tb01471.x. [DOI] [PubMed] [Google Scholar]
  8. Haukipuro K., Melkko J., Risteli L., Kairaluoma M., Risteli J. Synthesis of type I collagen in healing wounds in humans. Ann Surg. 1991 Jan;213(1):75–80. doi: 10.1097/00000658-199101000-00013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haukipuro K., Risteli L., Kairaluoma M. I., Risteli J. Aminoterminal propeptide of type III procollagen in healing wound in humans. Ann Surg. 1987 Dec;206(6):752–756. doi: 10.1097/00000658-198712000-00011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haukipuro K., Risteli L., Kairaluoma M. I., Risteli J. Aminoterminal propeptide of type III procollagen in serum during wound healing in human beings. Surgery. 1990 Apr;107(4):381–388. [PubMed] [Google Scholar]
  11. Heinrich P. C., Castell J. V., Andus T. Interleukin-6 and the acute phase response. Biochem J. 1990 Feb 1;265(3):621–636. doi: 10.1042/bj2650621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hämäläinen L., Oikarinen J., Kivirikko K. I. Synthesis and degradation of type I procollagen mRNAs in cultured human skin fibroblasts and the effect of cortisol. J Biol Chem. 1985 Jan 25;260(2):720–725. [PubMed] [Google Scholar]
  13. Kiistala U. Suction blister device for separation of viable epidermis from dermis. J Invest Dermatol. 1968 Feb;50(2):129–137. doi: 10.1038/jid.1968.15. [DOI] [PubMed] [Google Scholar]
  14. Lazarou S. A., Barbul A., Wasserkrug H. L., Efron G. The wound is a possible source of posttraumatic immunosuppression. Arch Surg. 1989 Dec;124(12):1429–1431. doi: 10.1001/archsurg.1989.01410120079015. [DOI] [PubMed] [Google Scholar]
  15. Melkko J., Niemi S., Risteli L., Risteli J. Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen. Clin Chem. 1990 Jul;36(7):1328–1332. [PubMed] [Google Scholar]
  16. Miller E. J., Gay S. The collagens: an overview and update. Methods Enzymol. 1987;144:3–41. doi: 10.1016/0076-6879(87)44170-0. [DOI] [PubMed] [Google Scholar]
  17. Moshage H. J., Janssen J. A., Franssen J. H., Hafkenscheid J. C., Yap S. H. Study of the molecular mechanism of decreased liver synthesis of albumin in inflammation. J Clin Invest. 1987 Jun;79(6):1635–1641. doi: 10.1172/JCI113000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oikarinen A., Autio P., Kiistala U., Risteli L., Risteli J. A new method to measure type I and III collagen synthesis in human skin in vivo: demonstration of decreased collagen synthesis after topical glucocorticoid treatment. J Invest Dermatol. 1992 Feb;98(2):220–225. doi: 10.1111/1523-1747.ep12555884. [DOI] [PubMed] [Google Scholar]
  19. Oikarinen A., Autio P., Vuori J., Vänänen K., Risteli L., Kiistala U., Risteli J. Systemic glucocorticoid treatment decreases serum concentrations of carboxyterminal propeptide of type I procollagen and aminoterminal propeptide of type III procollagen. Br J Dermatol. 1992 Feb;126(2):172–178. doi: 10.1111/j.1365-2133.1992.tb07816.x. [DOI] [PubMed] [Google Scholar]
  20. Raghow R., Gossage D., Kang A. H. Pretranslational regulation of type I collagen, fibronectin, and a 50-kilodalton noncollagenous extracellular protein by dexamethasone in rat fibroblasts. J Biol Chem. 1986 Apr 5;261(10):4677–4684. [PubMed] [Google Scholar]
  21. Risteli J., Niemi S., Trivedi P., Mäentausta O., Mowat A. P., Risteli L. Rapid equilibrium radioimmunoassay for the amino-terminal propeptide of human type III procollagen. Clin Chem. 1988 Apr;34(4):715–718. [PubMed] [Google Scholar]
  22. Scharffetter K., Kulozik M., Stolz W., Lankat-Buttgereit B., Hatamochi A., Söhnchen R., Krieg T. Localization of collagen alpha 1(I) gene expression during wound healing by in situ hybridization. J Invest Dermatol. 1989 Sep;93(3):405–412. [PubMed] [Google Scholar]
  23. Schulze S., Sommer P., Bigler D., Honnens M., Shenkin A., Cruickshank A. M., Bukhave K., Kehlet H. Effect of combined prednisolone, epidural analgesia, and indomethacin on the systemic response after colonic surgery. Arch Surg. 1992 Mar;127(3):325–331. doi: 10.1001/archsurg.1992.01420030095018. [DOI] [PubMed] [Google Scholar]
  24. Solis-Herruzo J. A., Brenner D. A., Chojkier M. Tumor necrosis factor alpha inhibits collagen gene transcription and collagen synthesis in cultured human fibroblasts. J Biol Chem. 1988 Apr 25;263(12):5841–5845. [PubMed] [Google Scholar]
  25. Stephenson M. L., Krane S. M., Amento E. P., McCroskery P. A., Byrne M. Immune interferon inhibits collagen synthesis by rheumatoid synovial cells associated with decreased levels of the procollagen mRNAs. FEBS Lett. 1985 Jan 21;180(1):43–50. doi: 10.1016/0014-5793(85)80227-1. [DOI] [PubMed] [Google Scholar]
  26. Weiner F. R., Czaja M. J., Jefferson D. M., Giambrone M. A., Tur-Kaspa R., Reid L. M., Zern M. A. The effects of dexamethasone on in vitro collagen gene expression. J Biol Chem. 1987 May 25;262(15):6955–6958. [PubMed] [Google Scholar]

Articles from Annals of Surgery are provided here courtesy of Lippincott, Williams, and Wilkins

RESOURCES