Skip to main content
. 2025 Aug 22;26(17):8149. doi: 10.3390/ijms26178149
Shape terminology
Hull the smallest convex shape that completely encloses the cell.
Endpoint The terminus or end of a microglial process or branch.
Junction The point at which the processes of a microglial cell divide or connect.
Slab voxels The segment of a microglial process between an endpoint and junction.
Feature terminology
Foreground pixels The pixels representing the cell after thresholding.
Maximum span across hull The longest distance between any two points on the convex hull of the cell.
Area The number of pixels that make up the cell.
Perimeter The length of the cell’s outer boundary.
Width of bounding rectangle The horizontal extent of the smallest rectangle that encloses the cell.
Height of bounding rectangle The vertical extent of the smallest rectangle that encloses the cell.
Maximum radius from hull’s center of mass The longest distance from the convex hull’s centroid (center of mass) to its outer boundary.
Mean radius The average distance from the cell’s center to its outer boundary.
Diameter of bounding circle The diameter of the smallest circle that completely encloses the cell.
Maximum radius from circle’s center of mass The longest distance from centroid to the cell’s boundary.
Mean radius from circle’s center of mass The average distance from the cell’s centroid to any pixel of the cell.
Density of foreground pixels in hull area The fraction of the convex hull that is filled by the cell’s pixels
Span ratio of hull (major/minor axis) The ratio of the convex hull’s longest axis to its shortest axis.
Circularity How circular the cell is on a scale of 0 to 1.0, where 1.0 is a perfect circle.
Max/min radii from hull’s center of mass The ratio between the longest and shortest distances from the convex hull’s centroid to its edge.
Relative variation (CV) in radii from hull’s center of mass The coefficient of variation of the distances between the convex hull’s centroid and outer boundary.
Max/min radii from circle’s center of mass The ratio between the longest and shortest distances from the bounding circle’s centroid to the cell’s outer boundary.
Relative variation (CV) in radii from circle’s center of mass The coefficient of variation of the distances between the bounding circle’s centroid and outer boundary.
# of branches The quantity of branch segments in the skeletonized structure.
# of junctions The quantity of points where two or more branches converge in the skeleton.
# of end point voxels The quantity of voxels at the ends of branches in the skeleton.
# of junction voxels The quantity of voxels that are part of a junction.
# of slab voxels The quantity of voxels that form the segments between junctions and endpoints in the skeleton.
# of triple points The quantity of junctions in the skeleton where exactly three branches meet.
# of quadruple points The quantity of junctions in the skeleton where exactly four branches meet.
Maximum branch length The length of the longest continuous branch in the skeleton.
Average branch length The mean length of all branches in the skeleton.