Table 1.
Release activity of fission yeast eRF1 and wild-type and mutant ΨeRF1 proteins in an in vitro RF assay
| eRF1 | f[35S]Met released, cpm
|
|||
|---|---|---|---|---|
| UGAA | UAGA | UAAA | UGGA | |
| Human eRF1 | 4840 | 4850 | 4340 | 0 |
| S. pombe eRF1 | 4000 | 3800 | 3700 | 0 |
| Hybrid ΨeRF1 | ||||
| Wild type (KATNIKD) | 4200 | 0 | 0 | 0 |
| Mutant (KASNIKD) | 4500 | 0 | 0 | 0 |
| Mutant (TATNIKD) | 4300 | 0 | 0 | 0 |
| Mutant (TASNIKD) | 3800 | 0 | 0 | 0 |
| Mutant (TASNIKS) | 2000 | 0 | 0 | 0 |
The background level varied from 600 to 1200 cpm depending on the eRF1 preparation present in the incubation mixture. Zero means that the amount of cpm was ±15% of the background level. Average values from three independent experiments are presented.