Figure 11.

Structural mapping of thermodynamically reclassified pathogenic CDKL5 variants within the kinase domain. Thermodynamically reclassified pathogenic variants are mapped on the CDKL5 kinase domain based on their relative impact on protein folding and binding stability. Variants exhibiting higher folding destabilization than binding destabilization (ΔΔG_Fmax > ΔΔG_Bmax) are annotated in red with transparent-surface rendering. Conversely, variants where binding destabilization is equal to or exceeds folding destabilization (ΔΔG_Fmax ≤ ΔΔG_Fmax) are annotated in blue.