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Expression of MGAT1 in mammalian cells. (a) Immunoblotting of FLAG-tagged proteins to verify expression of MGAT1 and control proteins. Cells were transfected with vector only or with FLAG-tagged versions of MGAT1, DGAT2, DGAT1, or ACAT2. (b) Immunocytochemistry of COS-7 cells expressing FLAG-tagged MGAT1. An anti-FLAG antibody and FITC-conjugated secondary antibody were used. (c) Acyltransferase activities in COS-7 cell membranes. [14C]oleoyl-CoA was used for enzyme assays.
