Anti-KLH IgG antibody titers (a), splenic CD4+CD8− T cell percentages (b), and relative blood lymphocyte percentages (c) in control and genistein-treated mice. (a) Juvenile (30 days old) ovariectomized mice were treated with DMSO or genistein for 5 weeks, and serum anti-KLH antibody was measured to determine whether genistein diminished antibody response. Results are expressed as a percentage of control. (b) Splenic CD4+CD8− T cell percentages in mice from a, measured by flow cytometry. (c) Relative blood lymphocyte percentages in mice shown in a and b. For all groups, n = 5–9. All genistein treatments were less than controls, but were not different from each other.