Figure 2.
Limiting dilution experiments demonstrate transplantability of BA+NH- and TP+NH-induced disease. (a and b) Kaplan–Meier survival analysis of secondary recipient mice. Secondary mice received 106, 105, 104, or 103 cells from the primary donor mouse. Eight recipient mice were used for dilutions 106–104, whereas four mice received 103 cells. As few as 104 spleen cells from a mouse with either (a) BA/NH or (b) TP/NH disease, when transplanted into secondary, sublethally irradiated recipient mice, was sufficient to recapitulate the primary disease. The histopathologic analysis of spleens from the secondary recipients was similar to that seen in primary transplant recipients [BA/NH (c), TP/NH (d)], consisting of a diffuse proliferation of myeloid blast forms with a myeloid maturation arrest.