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. 2002 Jun 25;99(13):8542–8547. doi: 10.1073/pnas.082241699

Table 2.

Kinetics of mutant and wild-type nucleases

Nuclease Catalytic parameters
kcat, min−1 Km, μM kcat/Km, μM−1⋅min−1 ΔΔGapp, kJ·mol−1
Wild type 101  ± 10 0.07  ± 0.01 1,400 —
R216A 97  ± 4 1.8  ± 0.3 54 8.4
K215A* 38 0.8 48 10
*

Result was reported previously (25).