Skip to main content
. 2002 May 21;99(11):7646–7651. doi: 10.1073/pnas.112039899

Figure 7.

Figure 7

Phagocytosis of serotype M1 GAS and colocalization of Sic with ezrin in human PMNs. Human PMNs were incubated with fluorescently labeled and opsonized MGAS5005 (black bars) or MGAS5005sic (white bars) and the amount of (A) phagocytosis, (B) ingested and bound bacteria, and (C) reactive oxygen species production was determined at the indicated times by flow cytometry. Results are the mean ± SD of three separate experiments. (D) Bactericidal assay. Human PMNs were incubated with MGAS5005 or MGAS5005sic, and bactericidal activity was determined after 2–3 h. Results are the mean ± SD of three separate experiments. *, P = 0.0004 vs. MGAS5005. (E–G) PMNs were treated with purified Sic1.01 for 5 min, fixed, and permeabilized. Double fluorescence labeling was conducted with goat anti-ezrin antiserum (green) and an affinity-purified rabbit polyclonal antibody to Sic (red). G is an overlay of the two images, with colocalization visualized as yellow.