Fig 5. Select lipid species in heads differentiate injured and uninjured flies.

Lipidomic analysis of heads was performed at 1, 7, 14, and 21 days post-injury of 7-day old, male, w¹¹¹⁸ flies to assess how injury alters the abundance of individual lipid species. PLS-DA analysis of injured and uninjured flies at (A) and (B) 1 day post-injury and (C) and (D) 7, 14, and 21 days post-injury. In scores plots (A) and (C), small colored circles indicate independent samples, and colored ovals encompassing circles for a given condition indicate the 95% confidence limit. Components 1 and 2 percentages represent the percentage of the total variance in the data that is explained by the component. In (B) and (D), Variable Importance in Projection (VIP), is a measure of how important each lipid species is in differentiating injured from uninjured flies. Heat maps show the relative abundance of each lipid species under the examined conditions: Red is high and blue is low abundance.