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Flowchart of the two independent searches for novel Hrp effectors. (A) ORFs with 5′ Hrp promoters were analyzed for horizontal transfer indicators to yield a set enriched in candidate effectors for secretion testing. (B) Valid ORFs found by the genomewide search for export signals were analyzed for indicators of horizontal acquisition and association with possible pathogenicity islands or islets to yield a set enriched in candidate effectors for secretion testing.
