Table 1.
ORFs with 5′ Hrp promoter sequences and encoding proteins demonstrated to be secreted by the P. syringae Hrp system
| Initial designation* | New designation | Size, bp | % G+C | Homolog (blastpE value) | GenBank accession no. and/or reference |
|---|---|---|---|---|---|
| ORF1 | HopPtoI | 1,899 | 48.9 | None | NA |
| ORF2 | HopPtoH | 657 | 47.2 | ORF3 from P. s. pisi avrPpiC2 locus (1e-114) | CAC16702 (26) |
| ORF3 | HopPtoE | 636 | 50.7 | None | NA |
| ORF4 | HopPtoG | 1,482 | 43.7 | Hypothetical protein from R. solanacearum (1e-137) | NP_521884 |
| ORF5† | HopPtoS1 | 852 | 46.5 | Chicken ADP-ribosyltransferase (1e-5) | P55807 (39) |
NA, not available.
*
Nucleotide sequences of all ORFs (including ORFs 6–8, which encode proteins that failed the secretion tests) are provided as supporting information on the PNAS web site.
†
Determined to possess an ART domain (pfam1129), further confirming its similarity to ADP-ribosyltransferases.