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. 2002 Jun 17;99(13):8579–8584. doi: 10.1073/pnas.122061399

Figure 1.

Figure 1

Reduction of ebselen by NADPH catalyzed by human TrxR. Concentrations of 10 μM (○, □) and 20 μM (●, ■) ebselen in 0.5 ml of 50 mM Tris⋅Cl/1 mM EDTA, pH 7.5 containing 100 μM NADPH was mixed with 10 nM (□, ■) and 50 nM (○, ●) human TrxR, and the A340 was followed against identical blank with enzyme but without ebselen.