Figure 6.

Reduction of H₂O₂ by human TrxR and the effect of ebselen and Trx. To cuvettes containing 50 mM Tris⋅Cl, 1 mM EDTA, pH 7.5, and 100 μM NADPH was added 0.5 mM H₂O₂ and 17 nM human TrxR (hTrxR) (●), 17 nM hTrxR plus 2 μM ebselen (▴), or 17 nM hTrxR plus 2 μM ebselen and 4.5 μM human Trx (hTrx) (■). The A₃₄₀ was determined against a blank with 17 nM TrxR but without H₂O₂.