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Kinetics of competence-induced release of cytoplasmic βgalactosidase to the external medium. Samples assayed for β-galactosidase activity were collected from an S. pneumoniae EK4166 culture every 5 min for a period of 60 min after competence induction. Closed squares, supernatants from a culture that received 250 ng/ml of CSP-1 at time 0; open squares, supernatants from a corresponding noncompetent culture.
