Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Relevant characteristics | Source/ref. |
|---|---|---|
| Strain | ||
| S. pneumoniae Rx | R36A derivative | 37 |
| CP1200 | Rx, but malM511 str-1 | 15 |
| CP1415 | CP1200, but comA− by insertion of the ermAM cassette into the ClaI site of comA | 14 |
| CP1500 | hex nov-r1 bry-r str-1 ery-r1 ery-r2 | 38 |
| R262 | R800, but negative for endogenous β-galactosidase activity (ebg−) | 19 |
| R319 | R800, but comE∷pXF518 by transformation with plasmid DNA | 19 |
| EK100 | CP1415, but egb− by transformation with genomic DNA from R262 | This work |
| EK4166 | EK100, but hirL∷pEVP3 by transformation with plasmid DNA | This work |
| EK4167 | EK4166, but NovR by transformation with CP1500 genomic DNA | This work |
| H1 | CP1415, but NovR by transformation with CP1500 genomic DNA | This work |
| H2 | H1, but comE∷pXF518 by transformation with R319 genomic DNA | This work |
| H3 | H1, but lytA∷pEVP3 by transformation with plasmid DNA | This work |
| Plasmid | ||
| pEVP3 | Nonreplicative vector (CmR, lacZ) | 18 |