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Changes in Gal11 mutant 17 (M17) and their effects on activation. (A) Gal11M17 bears the four changes indicated: the point mutations T322K, L501P, and D530V and the short deletion Δ443/470. (B) Mutations from Gal11M17 were introduced separately and in the indicated combinations into Gal11. The mutant Gal11 proteins, expressed from plasmids, were introduced into cells otherwise deleted for Gal11 and assayed as in the experiment of Fig. 1. β-gal, β-galactosidase.
