Figure 5.

DNase I footprinting of native BenM at benA. DNase I-cleaved DNA was labeled on the antisense (A) or sense (B) strand of the benA promoter region. The presence (+) or absence (−) of BenM (0.15 μM), benzoate, and muconate in the reactions is noted. Inducers were added at a concentration of 1 mM in each reaction. The BenM binding Sites 1, 2, and 3 are discussed in the text. (C) Nucleotides protected from DNase I digestion in both the absence and presence of inducers are indicated by solid brackets immediately above or below the benA sequence. Nucleotides protected from DNase I cleavage only in the absence of inducers are indicated by dotted brackets. Solid triangles show sites that were hypersensitive to DNase I digestion.